a) Isostichopus sp.
The average TL was 193 ± 52 mm (± SD), limit 380 mm and minimal 75 mm. These thinking varied depending on gender (+ = 195 ± 45 mm, > = 202 ± 52 mm and you can as opposed to gonad 179 ± 61 mm). People as opposed to a beneficial gonad was indeed significantly smaller compared to the brand new guys (letter = 221; K = 6.513; p = 0.039).
Length-volume shipments are unimodal having a peak regarding the set of 180 so you’re able to 215 mm (Fig 2A). The newest L50 try built on 175 mm (95% believe period: 174–176 mm) (Fig 2C). The smallest private having gonads is a masculine (110 mm).
Good and you will B shipping volume out of length and lbs Isostichopus sp.; Age and you can F shipping volume out-of length and you may pounds Isostichopus badionotus; C and D size and you will weight initially readiness Isostichopus sp.; Grams and you may H duration and you may lbs to start with readiness Isostichopus badionotus.
The common BW (± SD) is 179 ± 69 grams, limit 558 g and you will lowest 18 grams (+ = 187 ± 58 grams, > = 190 ± 71 grams and instead of gonad 154 ± 77 grams). Individuals without gonads was indeed decreased hefty than female and you may males (letter = 221; K = ; p = 0.003).
Regularity shipment of BW was unimodal with a top inside the all of the 153 to 198 grams (Fig 2B). Brand new W50 is mainly based from the 155 grams (95% depend on period: 154–156 grams) (Fig 2D). The tiniest private gathered with gonads is a male (39 g).
b) Isostichopus badionotus.
The average TL was 324 ± 70 mm (± SD), maximum 480 mm and minimum 110 mm (+ = 329 ± 55mm, > = 341 ± 64 mm and without gonad 240 ± 78 mm). The individuals without gonad were significantly smaller (n = 114; F2,111 = ; p = 0.001).
The exact distance-frequency delivery was unimodal, that have a highest regarding the directory of 355 to help you 390 mm (Fig 2E). The latest L50 is actually built from the 220 mm (95% trust period: 217–221 mm) (Fig 2G). The smallest individual accumulated with gonads is actually a male (210 mm).
The average BW was 628 ± 179 g, maximum 999 g and minimum 72 g (+ = 654 ± 152 g, > = 669 ± 145 g and without gonad 383 ± 200 g). The individuals without a gonad were significantly less heavy (n = 114; Fdos,111 = ; p = 0.001).
Regularity shipments from BW is unimodal that have a highest regarding the selection of 607 to help you 642 g (Fig 2F). The fresh new W50 are oriented in the 348 g (95% believe period: 344–350 grams) (Fig 2H). The littlest individual built-up which have gonads try a woman (363 g).
Both in ocean cucumbers the brand new gonad is including a couple tufts out of tubules having an excellent racemose looks located at new anterior stop of your coelom, hence concerned occupy all the perivisceral cavity during the phase of readiness (III). The color of the gonad out of one another sea cucumbers varied away from beige–whitish to ocher, rather than an obvious matchmaking anywhere between colour and gender. Only one case of hermaphroditism was observed in Isostichopus sp., in which 3% of your own gonad contains jizz seeking arrangement eЕџleЕџme and you may 97% oocytes.
Histology demonstrated cuatro of one’s 5 grade out of gonadal creativity suggested by Ramofafia et al. . Contained in this analysis the brand new indeterminate phase (I), where gametes do not but really has actually discussed cellular formations that succeed the distinction, was not seen. A description of histological attributes out-of gonad development are revealed about S1 Appendix.
4.5 Gonad List (GI)
In both morphotypes a reduced GI beliefs was in fact registered regarding the basic 1 / 2 of the season, towards the low values set for Isostichopus sp. (0.4 ± 0.2%) and also for I. badionotus (0.8 ± 0.6%). From Will get beforehand, a progressive increase are seen, getting its limitation worth inside August which have 4.eight ± 0.9% and you will 7.6 ± 0.9%, respectively (Fig 3A and you can 3B), coinciding with the escalation in drinking water temperatures in addition to start of this new rainy season (Fig 3C and you will three dimensional). Ranging from September and you will December this new GI decreased quickly, appearing big spawning, tape during the good GI of just one.5 ± 0.5% to possess Isostichopus sp. and you will step one.step three ± 0.4% to have I. badionotus.